dprA [2018-03-08 18:39:27]

conveys incoming ssDNA to [protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|RecA]; facilitates the displacement of both SSBs ([protein|search|SsbB ]and [protein|8DE39680CA7663803A107066FBE9AFFCC77DB72A|SsbA]), increases [protein|search|RecA ]nucleation onto SSB-coated ssDNA, and mediates DNA strand annealing, required to internalize and to recombine ssDNA with homologous resident duplex

locus: BSU16110

pI: 9.76

mw: 32.78 kDa

protein length: 297 aa

gene length: 891 bp

function: [category|SW 3.1.5|DNA repair/ recombination]

product: recombination mediator protein

essential: no

synonyms: smf

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination]
• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination] → [category|SW 3.1.5.6|Other proteins]
• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.7|Genetic competence]
• [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.3|Genetic competence]

Gene

Coordinates: 1,682,580 → 1,683,473

Phenotypes of a mutant

• strongly impaired genetic recombination [Pubmed|23779106]
• ''[gene|80E6F156764FF30300D034EE6FB44F2DB8338AF3|recO] [gene|FC53216F600994C862F0C0D017C3FA28EB75DCB6|dprA]'' double mutants have a strongly reduced chromosomal transformation rate [Pubmed|23779106,22373918]

The protein

Catalyzed reaction/ biological activity

• binds ssDNA, promotes displacement of [protein|8DE39680CA7663803A107066FBE9AFFCC77DB72A|SsbA] and [protein|1CDF658441061EA476E27C2E60DEE45C4F45AC15|SsbB] from ssDNA [Pubmed|23779106]
• provides [protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|RecA] access to ssDNA during chromosomal transformation (together with [protein|80E6F156764FF30300D034EE6FB44F2DB8338AF3|RecO]) [Pubmed|22373918]
• RecA-ATP in concert with [protein|FC53216F600994C862F0C0D017C3FA28EB75DCB6|DprA] and [protein|8DE39680CA7663803A107066FBE9AFFCC77DB72A|SsbA] catalyzes DNA strand exchange, with [protein|1CDF658441061EA476E27C2E60DEE45C4F45AC15|SsbB] as an accessory factor [Pubmed|25138221]

Protein family

• smf family (according to Swiss-Prot)

Structure

• [PDB|3UQZ] (aa 90 ... 310, from Streptococcus pneumoniae, 44% identity) [pubmed|22904190]

[SW|Localization]

• transiently localizes to the cell pole, and co-localizes with the DNA uptake machinery [Pubmed|17630974]

Expression and Regulation

Operons

• genes: [gene|D72175937B3956C2BC500FC00CEC8EC57A0A76A8|sucC]-[gene|5900CAC1CE367F33B267673A7FC210A3A269B30E|sucD]-[gene|FC53216F600994C862F0C0D017C3FA28EB75DCB6|dprA]-[gene|41199C76DF8CA804B7B8878D61228B9542A96AE4|topA]
  description: [Pubmed|22383849]

  regulatory mechanism

  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|12850135], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]

  regulation

  • repressed by glucose (2.4-fold) ([protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]) [Pubmed|12850135]
  • expression is heterogeneous [pubmed|29809139]

Biological materials

Mutant

• BKE16110 (Δ[gene|FC53216F600994C862F0C0D017C3FA28EB75DCB6|dprA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE16110 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATAGATTGACCTCCTTTT, downstream forward: _UP4_TGAATTATCGTTTGACAAAC
• BKK16110 (Δ[gene|FC53216F600994C862F0C0D017C3FA28EB75DCB6|dprA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK16110 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATAGATTGACCTCCTTTT, downstream forward: _UP4_TGAATTATCGTTTGACAAAC

Labs working on this gene/protein

• [SW|Peter Graumann], Freiburg University, Germany [http://www.biologie.uni-freiburg.de/data/bio2/graumann/index.htm homepage]

References

• 17630974,17803906,12421306,18045469,11948146,22373918,23779106,23440217,25138221,26786319,28618091,22904190